International Journal of Agronomy

Research Article

Characterization of Rhizobia Isolated from Tigray Soil and Assessment of Their Effect on Germination and Seedling Vigor of Wheat and Field Pea

Table 7

Effect of rhizobia isolates on field pea seed germination and 7-day-old seedling growth parameters.


Isolates	GP	SSL (cm)	SRL (cm)	SVI

SH1	97.3 ± 2.30^a	3.48 ± 0.21^efgh	2.62 ± 0.37^hij	610 ± 44.0^efg
SH2	93.3 ± 2.30^abcd	3.22 ± 0.28^fghi	2.78 ± 0.31^gh	572 ± 34.8^hi
SH3	80.0 ± 4.00^hi	2.44 ± 0.26^no	2.66 ± 0.31^hi	408 ± 27.7^qr
SH4	90.7 ± 2.30^bcde	3.04 ± 0.08^ijkl	2.16 ± 0.08^kl	472 ± 15.1^no
SH5	82.7 ± 2.30^gh	2.72 ± 0.14^mn	2.24 ± 0.42^jkl	424 ± 32.6^pq
KO1	84.0 ± 4.00^fgh	2.88 ± 0.13^jklm	1.64 ± 0.24^m	395 ± 16.4^qr
KO2	97.3 ± 2.30^a	4.34 ± 0.11^c	3.72 ± 0.29^d	792 ± 42.7^c
KO3	94.7 ± 2.30^abc	5.70 ± 0.29^b	3.42 ± 0.42^de	805 ± 60.2^c
KO4	69.3 ± 2.30^l	1.68 ± 0.19^p	1.84 ± 0.20^lm	261 ± 8.2^s
KO5	84.0 ± 4.00^fgh	2.74 ± 0.16^lmn	2.16 ± 0.15^kl	406 ± 32.1^qr
KO6	96.0 ± 4.00^ab	3.96 ± 0.15^d	3.16 ± 0.27^efg	707 ± 30.1^d
KO7	78.7 ± 2.30^hij	3.90 ± 0.15^d	2.98 ± 0.20^fgh	535 ± 37.8^jkl
KO8	88.0 ± 0.00^defg	4.46 ± 0.37^c	2.88 ± 0.35^gh	640 ± 35.6^e
TS1	93.3 ± 2.30^abcd	3.20 ± 0.28^ghi	3.46 ± 0.38^de	613 ± 29.3^efg
TS2	73.3 ± 2.30^jkl	2.80 ± 0.29^klm	2.68 ± 0.31^hi	411 ± 52.9^pq
TS3	94.7 ± 2.30^abc	2.22 ± 0.22^o	1.66 ± 0.16^m	378 ± 11.8^r
TS4	86.7 ± 6.11^efg	3.52 ± 0.31^ef	2.28 ± 0.52^ijk	508 ± 66.2^lm
ME1	89.3 ± 2.30^cdef	3.12 ± 0.32^ij	3.62 ± 0.40^d	595 ± 33.9^gh
ME2	80.0 ± 0.00^hi	3.18 ± 0.23^hij	3.72 ± 0.33^d	565 ± 36.1^hij
ME3	92.0 ± 4.00^abcde	4.00 ± 0.21^d	2.30 ± 0.38^ijk	570 ± 31.0^hi
ME4	76.0 ± 4.00^ijk	2.78 ± 0.25^lm	2.74 ± 0.44^gh	440 ± 8.2^op
ME5	89.3 ± 2.30^cdef	3.74 ± 0.29^de	2.18 ± 0.23^kl	528 ± 70.2^kl
TA1	94.7 ± 2.30^abc	2.62 ± 0.23^mn	3.34 ± 0.35^def	546 ± 45.3^ijk
TA2	90.7 ± 2.30^bcde	3.50 ± 0.36^efg	3.52 ± 0.40^de	607 ± 32.7^fg
TA5	88.0 ± 4.00^defg	3.74 ± 0.26^de	3.48 ± 0.27^de	628 ± 53.5^ef
TA7	93.3 ± 4.61^abcd	3.10 ± 0.26^ijk	2.56 ± 0.43^hijk	493 ± 20.6^mn
TA8	92.0 ± 6.92^abcde	3.10 ± 0.24^ijk	4.30 ± 0.35^c	690 ± 9.7^d
TA9	93.3 ± 2.30^abcd	4.54 ± 0.32^b	5.32 ± 0.39^a	958 ± 42.6^a
TA10	93.3 ± 4.61^abcd	3.48 ± 0.32^efgh	4.78 ± 0.23^b	788 ± 73.1^c
NC	72.0 ± 4.00^kl	1.46 ± 0.15^p	2.20 ± 0.15^jkl	264 ± 28.0^s
PC	97.3 ± 2.30^a	5.70 ± 0.33^a	3.50 ± 0.47^de	918 ± 55.3^b
LSD (0.05)	5.48	0.31	0.42	31.71
SEm (±)	0.60	0.04	0.06	3.48
F-probability	<0.001	<0.001	<0.001	<0.001
CV (%)	3.81	7.47	11.43	3.43

Germination and shoot/root data are means of 3 replications and each replicate of the shoot/root length was an average of 10 field pea seedlings per petri-plates harvested after 7 days. Means in the same column that are followed by different letters are significantly different at using Fisher’s LSD test; LSD: least significant difference; CV: coefficient of variation (%); SEm: standard error of mean; NC: negative control; PC: positive control; GP: germination percentage; SSL: seedling shoot length; SRL: seedling root length; SVI: seedling vigor index.